Watson sat out the 24-17 defeat to France in Paris with the same issue and it was hoped he would return for the trip to Murrayfield.
“Anthony felt it again yesterday and we won’t consider him for Scotland,” forwards coach Matt Proudfoot said.
Last week Watson wasn’t be considered for selection due to a calf injury he was carrying before coming into camp.
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Watson, one of England’s top performers at the World Cup in Japan, limped out of Bath’s 25-19 Champions Cup defeat against Harlequins in early January.
He went off nursing a leg problem 11 minutes into the second-half.
And the Bath fullback underlined his frustration in front of watching England head coach Eddie Jones by hurling his gum-shield to the ground before leaving the action.
Bath DoR Stuart Hooper played down the injury at the time.
Watson joins Manu Tuilagi as one of two England attacking threats that will be forced to sit out England’s showdown with Scotland in the Scottish capital.
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Yesterday it was revealed Tuilagi will miss the match due to a “low-grade” groin strain but England head coach Eddie Jones is confident the centre’s tournament is not over.
The Leicester midfielder was retained on Monday in a 34-man training squad to prepare for this weekend’s game at Murrayfield despite having limped off 16 minutes into Sunday’s 24-17 defeat to France in Paris.
Jones confirmed on Tuesday that Tuilagi will not be available in Edinburgh, but is hopeful the 28-year-old will be back for the rest of the championship.
“Manu had his MRI scan (on Monday night) and he has a slight, low-grade adductor strain,” the Australian said. “We’re hopeful he’ll be fit for Ireland. It’s very good news, outstanding news.”
Press Association/additional reporting RugbyPass
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